Generator

Part:BBa_I738001:Design

Designed by: Kari Clase   Group: iGEM07_Purdue   (2007-05-26)


generates the feruloyl-CoA hydratase for vanillin


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 875
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 875
    Illegal NotI site found at 819
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 875
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 875
    Illegal NgoMIV site found at 248
    Illegal NgoMIV site found at 266
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Strong ribosome binding site, well documented promoter, strong terminator


Source

sdd Biobrick I738002

References